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Genetics delay ripening, control post-harvest loss

June 14
Times of India

NEW DELHI -- Scientists have isolated a gene part, called promoter, from tomato plant and hope to use it in modified form for delaying fruit ripening by a technology that can be extended to several other perishable fruits and vegetables.

The country does not have proper cold storage and refrigerated transport facilities which causes wastage of almost twenty per cent of all perishable fruit and vegetables due to rotting, Dr Kailash Bansal, principal scientist at Indian Agriculture Research Institute (IARI), the institute which accomplished the task, told PTI.

Elaborating on the new technique called the antisense RNA technology, he said, in it the pathway by which a fruit ripening hormone called ethylene is produced by plants, is blocked.

"For this, we have recently isolated a promoter which is needed for functioning of the gene that produces an enzyme called ACC synthase. This enzyme is required in an important step for ethylene synthesis," he said. The ACC synthase producing gene was isolated about two years ago.

The antisense RNA technology involves introduction of the gene and its promoter in reverse form in tomato plant cells. The product of reverse gene blocks the product of the normal gene, thus preventing production of ACC synthase enzyme.

This genetic technology would help in reducing post harvest losses and boost exports by giving the plants an inherent capacity to delay ripening, Bansal said, adding though non-genetic methods are in use to delay ripening, these are not feasible on large scale.

However, not all the products of normal gene are blocked and some amount of enzyme is still produced, he said. But, as ethylene is not produced in sufficient quantity, ripening does not take place at normal rate and is slowed down, Bansal said.

A similar method is being tested in US, where scientists are using the gene promoter from their indigenous plants, he said, adding in that method, ethylene needs to be supplied exogenously.

"In India, we are trying to regulate ethylene synthesis so that ripening is slowed down but exogenous ethylene is not needed," he said. Earlier, Indian scientists had been working with an imported promoter, "but under the WTO it is better that we have our own promoter for commercializing."

"So far we have succeeded in introducing the gene and its promoter in reverse form in tomato seedlings. Molecular analysis and testing is on to analyze the status of the transformed plants," he said, adding it would take less than two years to transfer the technology to field trials.

One problem is that as gene is introduced using a bacterium (agrobacterium), a bacterial gene responsible for antibiotic resistance is also transferred in plant cells, he said.

Though, this gene does not have any effect on humans, efforts are on to find some other bacterial marker, he said. The technology has an advantage over the methods like early plucking as tomato fruits do not ripen at the same time and need plucking three-four times a year.

The new technology, which can be extended to mangoes and bananas, would be more economical and give plants uniformity in terms of ripening. It would also enhance their nutrient content.


Genetically engineered corn cleared in 17 food reactions 

Product did not trigger allergies, health officials report

June 14
Washington Post

Genetically engineered StarLink corn did not cause allergic reactions in 17 people who had reported sometimes severe reactions after eating corn tacos and tortillas last fall, federal health officials said yesterday.

Blood tests failed to find any signs of antibodies to the protein in the genetically engineered corn, indicating none of those tested had experienced an allergic reaction, the federal Centers for Disease Control and Prevention said. All had complained to federal agencies last year of reactions ranging from rashes to anaphylactic shock after eating products made of yellow corn that might have contained StarLink.

The results were applauded by advocates of biotechnology as confirming the safety of StarLink in particular and modified crops in general. But environmental groups called the federal effort limited and insufficient to answer the question of whether StarLink can cause dangerous allergic reactions.

Carol Rubin, an epidemiologist with the CDC's National Center for Environmental Health, said that while the test results were "only a first step" in answering questions about the safety of the corn, "it can be seen as good news for consumers."

"Based on the test methods used, it is highly unlikely that the people had an allergic reaction to" StarLink, she said. "They may have experienced allergic reactions to some food, but not the protein that was tested."

But one of the people who suffered anaphylactic shock after eating an enchilada made of yellow corn, Californian Grace Booth, said she was still convinced she had a reaction to StarLink.

"Everything else I ate in the 72 hours before I got so sick, I've eaten again with no problem," she said. "Frankly, I don't trust the tests."

StarLink is a variety of corn engineered to contain a protein, called Cry9c, that can protect crops against several insects. While many similar modified corns have been approved for general use, StarLink was approved only for animal feed because of concerns that it broke down more slowly than many proteins and might cause allergic reactions. The corn nonetheless inadvertently entered the human food supply, triggering the recall of about 300 corn products.

The developer of the corn, Aventis CropSciences of Research Triangle, N.C., has asked the Environmental Protection Agency for retroactive approval to allow small amounts of StarLink corn in food for people. The tests results announced yesterday were requested by a scientific advisory panel convened by the EPA to help determine whether to issue that approval. The advisory panel is set to meet again on the StarLink issue next month.

Val Giddings of the Biotechnology Industry Organization said that yesterday's results meant that the case was "slam-dunk closed."

"We are pleased, but not the least bit surprised, that the data released by the CDC today is consistent with the vast body of data we have had all along showing the safety of StarLink corn," he said. "If the protein was allergenic, they would not have found these negative reactions."

The tests were done at a Food and Drug Administration laboratory, and required new research into how to test for possible reactions to foods containing genetically modified crops. The blood samples, which included some taken before StarLink was developed and some from highly allergic people, were sent with number codes to prevent bias.

Rebecca Goldburg, a scientist with Environmental Defense, said that the CDC sample was too small to be meaningful, and that the EPA should not approve StarLink for human use based on the results. She said a wider test of people, especially children and those who eat enough yellow corn products to develop a sensitivity, is needed.

"The results are comforting, but hardly definitive," she said. "This is not something that could be published in a scientific journal and be accepted as conclusive. They didn't target the right people to test."

Mark Helm of Friends of the Earth, an environmental group that first brought the issue of StarLink in taco shells to public attention, said it was "borderline irresponsible to say this stuff is safe. It still has not been rigorously tested."

According to the CDC's Rubin, 51 people had reported experiencing adverse reactions in the weeks after StarLink was found in tacos and other corn products.

Of those, she said, 28 fit the definition of having suffered a food allergy, and 17 of them agreed to provide a blood sample. Some of the reactions had been quite severe, and Rubin said that all will receive reports on what the researchers learned about their blood.

Allergy experts generally agree that if antibodies to StarLink had been found in the blood of the 17, that still would not have meant the people had allergic reactions to the modified corn. That determination could be made only after completing skin prick tests or food challenges.

Booth, 35, who works at a youth center, said that she remained willing to have a skin test with StarLink protein or even eat something made with StarLink under a doctor's supervision. "I still feel like I haven't gotten to the bottom of this, and very much want to do that," she said.


U.S. finds no allergies to altered corn

June 14
New York Times

Government scientists said yesterday that they had found no evidence that any people had had allergic reactions to the genetically modified StarLink corn.

The findings could dispel public concern that the corn, which spread through the nation's food supply even though it was never approved for human consumption, represents a threat to public health.

The results, announced by the Centers for Disease Control and Prevention, could also clear the way for the Environmental Protection Agency to permit small amounts of the corn to be present in food without leading to recalls, something that would greatly relieve the farmers, grain processors and food companies.

The C.D.C. and the Food and Drug Administration tested the blood of 17 people who had reported suffering allergic reactions ranging from upset stomachs to potentially fatal anaphylactic shock after eating products they thought contained StarLink corn.

The agencies looked to see if the blood contained antibodies to the protein in the corn that was considered the possible allergen. No such antibodies were found, an indication that none of the people had had immune reactions to the corn protein.

"We do not have any positive results whatsoever," said Carol S. Rubin, a research scientist at the National Center for Environmental Health at the C.D.C. Still, the report prepared by the centers says that there is some possibility that people could be allergic without having antibodies in their blood.

StarLink contains a bacterial gene that permits the corn to produce a protein that kills the corn borer, a major pest. But that protein, known as Cry9C, has some characteristics of an allergen, mainly that it is not digested easily in the stomach.

Because of those concerns, the corn was approved for use as animal feed but not for human consumption. But it leaked into the human food supply, causing huge recalls of taco shells and other products, hurting American grain exports, forcing grain elevators and food companies to test incoming shipments of corn, and raising new concerns about the possible health effects of genetically engineered foods.

Now, the biotechnology industry, which said all along that there was little threat to public health from the corn, may breathe a bit easier. "We are pleased, but not the slightest bit surprised," said L. Val Giddings, vice president for food and agriculture of the Biotechnology Industry Organization, a trade group.

Critics of genetically modified food said the testing was insufficient. "These tests are not proof that StarLink corn is safe for human consumption," said Matt Rand, a spokesman for Genetically Engineered Food Alert, a coalition of environmental groups. "This is not a thorough investigation."

He said the government did not test the hundreds of people who complained to food companies about reactions instead of to the F.D.A. He also said it appeared that the test might have been performed using Cry9C made by bacteria, which might be different from the protein made in the corn.

Dr. Rubin said she could not comment on the specifics of the antibody test because it was designed by the F.D.A. An F.D.A. spokeswoman said her agency would not comment.

Dr. Marc E. Rothenberg, the section chief for allergy and immunology at Children's Hospital in Cincinnati and an adviser to the E.P.A. on this issue, said that antibody tests were more likely to report false positives than false negatives. Just because a person has antibodies to a protein does not mean she suffered an allergic reaction, he said. But if there are no antibodies, he said, there is only a tiny chance that the person had an allergic reaction.

The results of the blood tests will be used by the E.P.A. to decide whether to permit small amounts of StarLink in human food. Aventis CropScience, the company that developed StarLink, has asked the agency to permit such a small presence for four years, so the food industry would be spared the costs and disruptions of testing and food recalls. The E.P.A. has scheduled a meeting of its scientific advisory panel for next month to discuss the issues.

Aventis , which declined to comment on the results announced yesterday, has asserted that since most of the StarLink grown has been collected by the company and the government, there is so little in the food supply that it would not cause any public health problems even if Cry9C were found to be an allergen.

The C.D.C. and the F.D.A. reviewed the cases of 51 people who had told the agency about allergic reactions they thought were connected to StarLink. Of those, 28 people were considered to have had some sort of allergic reaction, while the rest were judged to have had some other health problem.


U.S. finds no link in biotech corn, illness

June 14
Boston Globe

Tests carried out by the Food and Drug Administration and the Centers for Disease Control have cast doubt on claims by dozens of people that they became sick after eating a genetically engineered corn not approved for human consumption.

The results, released yesterday, found no evidence that sensitivity to a protein known as Cry9c contained in StarLink corn caused allergic reactions, which reportedly ranged from itching to shock, the CDC said in the highly anticipated report.

Although not definitive, the government's findings were hailed by the biotechnology industry and could mark a first step in ending the controversy that erupted when StarLink was first discovered in a sample of Taco Bell taco shells last year. Its presence prompted more than 300 recalls of everything from chips to corn dogs, slowed grain exports, particularly to Asia, and threw part of the country's agricultural industry into chaos.

Aventis SA, the French pharmaceutical company that invented the variety and had earlier complained that the government's tests would not be conclusive, declined to comment on the results. But the Biotechnology Industry Organization, a trade group in Washington, said the findings should be enough ''to put concerns to rest.''

''As a scientist, there are always questions I'd like to ask,'' said L. Val Giddings, the group's vice president for food and agriculture. ''But from a standpoint of what we need to know from a public health point of view, this should be case-closed.''

Critics of the biotech industry said they thought the study was far too limited. They contended that more people should have undergone tests and that the government's scientists should have employed better methods, including skin-prick tests.

''This test does not prove that StarLink corn is safe for human consumption,'' said Matt Rand, campaign manager for biotech at the National Environment Trust, a lobbying group that opposes bioengineered foods. ''They chose only to test a handful of people. This is not a representative sample of the population that was exposed to StarLink corn.''

The danger posed by StarLink, which the government had approved only for animal consumption, has been a matter of fierce debate.

More than 50 people had reported allergic reactions - diarrhea, vomiting, stomach aches, and shock - after eating products that contained the corn.

Critics also have cited a report by a panel of independent scientists in December to the Environmental Protection Agency. The report said that StarLink shows a ''medium likelihood'' of causing an allergic reaction in some people. That same report, however, said the low levels of the protein likely present in food probably would not make people sick.

CDC officials stopped short of calling yesterday's results the final word. Even the report acknowledged that the tests on blood samples, which did not detect antibodies identifying an allergy, could not rule out the possibility of a reaction.

''It was a first safe step,'' said Dr. Carol Rubin, an epidemiologist with the CDC's Center for Environmental Health.

The results were eagerly anticipated because the EPA is considering a request by Aventis to allow a trace amount of StarLink in foods for humans. The EPA said yesterday that while the results were important, it would make no decision on Aventis' request until after a July 17-18 meeting of its scientific advisory panel.


U.S. watchdog group assails genetic field testing

June 14
Reuters

Boston -- A consumer watchdog group on Thursday accused the U.S. Department of Agriculture (USDA) of rubber stamping approval of field tests of genetically engineered crops that could harm people and the environment.

``Our environment is serving as the laboratory for widespread experimentation of genetically engineered organisms with profound risks that, once released, can never be recalled,'' the U.S. Public Interest Research Group said in a 98-page report called ``Raising Risk.''

The Washington-based group said the USDA authorized nearly 29,000 field tests of genetically engineered organisms through 2000. The agency only has rejected 4 percent of the applications to conduct field tests, the report stated.

The experiments were being done in an open environment with little oversight or public notification, the watchdog group said. Introducing nonnative organisms into the environment is estimated to cost the United States $123 billion annually in ecosystem damage, it added.

``Contrary to popular belief, the technology is not very precise,'' the report said. ``Scientists cannot control the location where the gene is inserted into the host's genetic code, nor guarantee stable expression of the gene in the new genetically engineered organism.''

The experiments sometime deal with bizarre combinations, such as introducing tomato genes to fish genes, or even combining pig genes with human genes, according to the report.

Between 1987 and 2000, leading U.S. biotech firm Monsanto Co. applied to conduct the most field tests every year.

Hawaii has hosted the most field tests with 3,275 and Illinois ranked second with 2,832, according to the study.

Concerns about genetically modified food reached a frenzy in the United States last year when StarLink, a genetically modified corn which has been approved for animal feed but not for human consumption, was discovered in a number of food products. StarLink is made by the Franco-German pharmaceutical group Aventis .

A report from the U.S. Centers for Disease Control and Prevention released on Wednesday found the biotech corn that spawned a nationwide recall last fall did not cause the allergic reactions that people reported after finding out about the grain.

U.S. PIRG recommends a moratorium on field testing and commercialization of genetically engineered foods and crops until it can be determined they do no harm the environment or people.

The demand for testing genetically modified plants surged in the 1980s during the U.S. biotech boom.

The U.S. PIRG report says corporations and universities are becoming increasingly secretive about their genetic testing. From 1989 to 1999, the percentage of crops containing genes declared confidential increased nearly every year. Last year, 65 percent of the genetically engineered crops were declared ``confidential business information,'' the study said.


Feature: Argentine farmers bet on biotech

June 14
Reuters

Buenos Aires -- Genetically modified (GM) soybean seeds have made Argentine farmer Hector Salmoiraghi's life a lot easier, he says as he stoops to examine a soybean plant in a vast field lined with eucalyptus trees.

Salmoiraghi, whose father and grandfather were also farmers, has been growing soybeans for 25 years, outside the port city of San Pedro, some 124 miles (200 km) northwest of Buenos Aires.

``Genetically-modified soy simplified everything,'' he said, adjusting his black gaucho-style beret and standing tall in his rubber boots and Wrangler jeans.

Salmoiraghi began using U.S.-based biotechnology giant Monsanto's Roundup Ready (RR) soybean seeds five years ago.

Made to be resistant to the company's powerful Roundup weed killer, the seeds cut costs by an estimated $50-$60 per hectare (2.47 acres) by reducing the preparation needed before planting.

Salmoiraghi, who planted 250 hectares (617.5 acres) with GM soy this season and plans to plant more next year, says he'd never go back to the traditional seeds: ``It wouldn't make sense. GM soy is much easier to work with.''

He is one of thousands of Argentine farmers singing the praises of GM products, and now the government is behind them more than ever before.

Argentina, a major grain and oilseed producer, is second only to the United States in the use of biotechnology products and has recently re-dedicated itself to promoting their development and attracting biotech companies that want to invest here.

After 3 years of a de facto moratorium on approving GM products, Argentina in May authorized the use of Monsanto's RR cotton.

Weeks later, the government created a biotechnology commission and Agriculture Secretary Marcelo Regunaga set off for the United States, where he met with biotech companies and visited research centers.

``We share the biotechnology policy of the U.S.,'' said Regunaga in a recent interview.

``I told (U.S. Agriculture) Secretary (Ann) Veneman that in all the international forums, we need to present a common position in which Argentina would have a more aggressive attitude than it has had in the past.''

Argentina is betting that biotechnology is the wave of the future. But sharing a common policy with the United States also means the countries could share common foes.

ANTI-GM GROUPS

While proponents say GM products increase efficiency, environmental and consumer groups say the products are not well regulated and could contain hidden health and environmental risks.

``We are against the sale of these products until there is conclusive evidence of the long-term effects they could have,'' said Karla Irigoyen, the Chile-based representative of watchdog group Consumers International.

``There is a lot of concern about the use of GM products in Argentina, not only for the sake of Argentine consumers but also for consumers in all of Latin America,'' where Argentina sends exports, said Irigoyen.

The government is well aware that GM products are controversial and will step up measures to ensure that no unapproved products are used illegally and that approved products are safe, said Regunaga.

Also approved in Argentina are insect-tolerant corns made by Swiss company Novartis and Monsanto, a herbicide-tolerant corn made by Franco-German company Aventis and an insect-tolerant cotton made by Monsanto.

THE TRADE ISSUE

Argentina's renewed commitment to biotechnology means that GM products are here to stay, at least for the time being. But whether GM is the future will depend on the impact the use of these products has on trade.

Agricultural shipments make up about 60 percent of Argentina's exports and GM products are a particularly dicey topic in the European Union and Asia, where imports of some products have been restricted on concern they may not be safe for human consumption.

``By approving (GM products) Argentina is definitely moving in the wrong direction and is risking its exports,'' said Emiliano Ezcurra, the coordinator of the biodiversity campaign for the Buenos Aires office of global environmental group Greenpeace.

Argentina sent nearly 30 percent of its grain exports and about 60 percent of its vegetable oil exports to Asia in 2000, according to data from the Agriculture Department.

China last week ruled that all production and sales of GM foods, including imports, must have government approval certifying that they do not cause any harm to humans, animals or the environment. It remains to be seen how the move will affect Argentine exports.

The European Union, where Argentina shipped about 10 percent of its grain exports and 60 percent of grain byproducts in 2000, has not approved any new GM crop varieties since 1998.

The European Parliament in February approved strict rules to regulate GM organisms, but France and five other countries said they would continue to block new GM permits until further rules are put in place ensuring gene-altered products can be traced back to their source.

``In Europe, it's a question of time,'' Carlos Popik, president of the Argentine unit of Monsanto, told Reuters in a recent interview.

``The question is purely political,'' he said. ``Europe prefers to increase production by subsidizing it instead of making it more efficient. These are the types of things that will be changed by time.''

Regunaga seemed unconcerned about Europe: ``In Europe there are people in favor of and people against'' biotechnology, he said. ``Certainly, we are going to try to find our friends in Europe so that we can have a more aggressive international position in favor of biotechnology.''

Politics aside, Argentine farmers will likely continue to support development of GM products that cut costs.

Salmoiraghi says RR soy has helped farmers survive in San Pedro, as lower global commodity prices, high interest rates and fuel prices and tax pressure have sent many small producers packing.

``GM soy has helped us,'' he said. ``It hasn't helped as much as it could have because prices have fallen, but it has certainly helped because with these fuel prices, I'd say that even we medium-sized producers wouldn't be around anymore.''


Organic food booms in Italy but sales still modest

June 14
Reuters

ROME -- Demand for organic food is exploding in Italy, but a hard core of consumers remains to be persuaded--with one in four Italians still unaware of what it is, Italy's biggest farmers' group said on Thursday.

Italian shoppers, proud of their country's culinary tradition and its renowned pasta, ham, cheese and wine, are increasingly turning to organic olive oil, rice, fruit and vegetables.

Research by Coldiretti showed the Italian market for organic food was growing by 20% a year and accounted for 0.6% of total food sales in 2000. Total organic food sales in Italy were 1.5 trillion lire ($660.6 million) in 2000.

Demand for organic produce is growing sharply across western Europe as consumers, worried about food scares and genetically modified foods, seek guarantees of food safety.

According to a recent survey by the farm ministry, most Italians oppose genetic engineering of their foods because of health worries.

``Demand for organic food is exploding, but one Italian in four does not know what it is,'' Coldiretti said on its web site.

The typical profile of a consumer of organic food in Italy was a single male graduate living in the wealthy north of the country, aged between 40 and 49, who had a well-paid job, Coldiretti said.

Many Italian supermarkets have special sections reserved for organic fare, which costs more than conventional foods.

``The survey of a representative sample of the entire Italian population reveals a worrying lack of awareness and information about organic produce due in part to unclear and incomprehensible labeling,'' Coldiretti said.

Coldiretti's president Paolo Bedoni urged strict standards for labeling of organic foods so that consumers were correctly informed about produce.

``It is necessary to implement measures to protect consumers and farmers from products entering the market that do not have the necessary guarantees,'' he said.

The Coldiretti research echoes the results of a recent poll by farm research body ISMEA, which said Italian shoppers, influenced by food scares, were increasingly turning to organic foods but that their awareness of such produce was patchy.

According to the poll of supermarkets and hypermarkets, 65% said Italian demand for organic foods was growing while 67% said consumers were poorly informed about it.

Italy is the ``most organic'' country in the EU, according to Coldiretti, with almost 50,000 companies engaged in producing organic food-40% of the EU total--and an organic farm area of almost one million hectares.

This represents more than a quarter of the total EU organic farm area.

Organic cereals--mainly durum wheat for pasta making, followed by barley, soft wheat and rice--represent between 23 and 25% of total organic agricultural production in Italy, according to Coldiretti.

Production of organically grown cereals is mainly in northern and central Italy. Farming of organic fruits and vegetables is predominantly in the south.

Analysts expect the new center-right government of Silvio Berlusconi, sworn into office this week, to encourage organic farming in Italy, which has an ideal climate for growing many types of organic produce.


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